Soluble E-Selectin ELISA Kit

Produktnummer ABIN2042658

Produktdetails Soluble E-Selectin ELISA Kit

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 5-8000 pg/mL

Untere Nachweisgrenze: 5 pg/mL

Applikation: ELISA

Marke: BioAssay™

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 4 pg/mL

Bestandteile: 96-well plate pre-coated with anti-sE-Selectin, Human
Lyophilized recombinant sE-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human E-Selectin in cell culture supernates, serum and plasma (heparin, citrate).E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4 The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Soluble E-Selectin

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Andere Bezeichnung: sE-Selectin (sE-Selectin Produkte)

Synonyme: CD62E ELISA Kit, E-selectin ELISA Kit, Elam ELISA Kit, selectin E ELISA Kit, selectin, endothelial cell ELISA Kit, Sele ELISA Kit

Gen-ID: 6401

UniProt: P16581