Neurotrophin 3 ELISA Kit

Produktnummer ABIN6719811

Hersteller Produkt- Nr.: koa0281

Das Ratte Neurotrophin 3 ELISA Kit (ABIN6719811) ist ein Colorimetric ELISA Kit zur Detektion von Ratte Neurotrophin 3.

Kurzübersicht für Neurotrophin 3 ELISA Kit (ABIN6719811)

Target: Neurotrophin 3 (NTF3)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Neurotrophin 3 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Hersteller Produkt- Nr.: koa0281

Hersteller: Rockland

Verwendungszweck: Rat Neurotrophin-3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: Y139-T257

Kreuzreaktivität (Details): There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

Sensitivität: < 2 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants or serum to each empty well. It is recommended that each rat Neurotrophin-3 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 5.5%, 7.2%, 5.5%
Inter-Assay Precision (CV%): 7.3%, 9%, 5.5%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Andere Bezeichnung: Neurotrophin-3

Hintergrund:

Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Gene Full Name: neurotrophin 3

Gen-ID: 81737

NCBI Accession: NP_001257797

UniProt: P18280

Pathways: RTK Signalweg, Neurotrophin Signalübertragung