Neurotrophin 3 ELISA Kit

Produktnummer ABIN411339

Das Maus Neurotrophin 3 ELISA Kit (ABIN411339) ist ein Colorimetric ELISA Kit zur Detektion von Maus Neurotrophin 3. Das Kit wurde in 2 Publikationen zitiert.

Kurzübersicht für Neurotrophin 3 ELISA Kit (ABIN411339)

Target: Neurotrophin 3 (NTF3)

Bindungsspezifität: AA 139-257

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Neurotrophin 3 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Mouse Neurotrophin-3 ELISA Kit to quantitate Mouse Ntf3 in cell culture supernatants and serum.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: Y139-T257

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

Sensitivität: < 2 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.4%, 4%, 5.6%
Inter-Assay Precision (CV%): 9.2%, 4.5%, 6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Neurotrophin 3 ELISA Kit (ABIN411339)

Wang, Li, Kong, Zhao, Yang, Chen, Yan: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, (2015) (PubMed).

Zhao, Wang, Peng, Ren, Zhang, Guo, Xu, Lu: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, (2014) (PubMed).

Details zu Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Andere Bezeichnung: NTF3

Hintergrund:

Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Gene Full Name: neurotrophin 3

Gen-ID: 18205

UniProt: P20181

Pathways: RTK Signalweg, Neurotrophin Signalübertragung