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ADAM8 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ADAM8.
Rockland
Produktnummer ABIN6719690
Hersteller Produkt- Nr.: koa0372

Kurzübersicht für ADAM8 ELISA Kit (ABIN6719690)

Target

Alle ADAM8 ELISA Kits anzeigen
ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Reaktivität

  • 5
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5 pg/mL - 4000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    62.5 pg/mL

    Hersteller Produkt- Nr.

    koa0372

    Hersteller

    Rockland

    Verwendungszweck

    Human ADAM8 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human ADAM8. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: M1-P497

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle ADAM8 ELISA Kits anzeigen

    ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

    Andere Bezeichnung

    ADAM8

    Hintergrund

    Synonyms: A Disintegrin And Metalloproteinase domain 8, ADAM 8, ADAM 8 precursor, ADAM metallopeptidase domain 8, Adam8, ADAM8_HUMAN, CD 156, CD156, CD156a, CD156a antigen, CD156a antigen, Cell surface antigen MS2, Cell surface antigen MS2, Disintegrin and metalloproteinase domain-containing protein 8, Human leukocyte differentiation antigen, Human leukocyte differentiation antigen, Macrophage cysteine rich glycoprotein, MGC134985, MS 2, MS2

    Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion during neurodegeneration.

    Gen-ID

    101

    NCBI Accession

    NP_001100

    UniProt

    P78325

    Pathways

    Activation of Innate immune Response, M Phase
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