IL-2 ELISA Kit

Produktnummer ABIN577087

Human IL-2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IL-2.

Kurzübersicht für IL-2 ELISA Kit (ABIN577087)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktdetails IL-2 ELISA Kit

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-2 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(03) IL-2 3 _x000C_ LIMITATIONS OF APPLICATION The Human IL-2 ELISA kit is not for use in clinical diagnostic procedures, and for laboratory use only. Although all manufacturing precautions have been exercised to ensure that this product will be suitable for use with all validated sample types as designated in the product insert, the possibility of interference cannot be excluded due to the variety of proteins that may exist within the sample. The Calibrator Diluent selected for the standard curve should be consistent with the assay samples. If the values generated by the samples are greater than the uppermost standard, the samples dilution should be adjusted with the appropriate Calibrator Diluent and the assay should be repeated

Analytische Methode: Quantitative

Sensitivität: The minimum detectable quantities of human IL-2 as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 5.7 pg/mL and 7.1 pg/mL respectively. The two standard deviations above the mean optical density of the 20 replicates of the zero standard were defined as the minimum detectable quantities.

Bestandteile: Standards: 1 set/2 vials

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Without preservative

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: Interleukin-2 (IL-2)

Hintergrund: Tumour Necrosis Factor alpha (TNF-alpha), also known as cachectin was initially named for its remarkable ability to cause hemorrhagic necrosis of tumours in mice.1 The TNF- gene located on chromosome 6 encodes a 233 amino acid (a.a.) prohormone bound to the plasma membrane, with the mature form (157 a.a., 17 kDa) exposed on the extracellular surface. Soluble, mature TNF- is released upon cleavage of the C- terminal.2,3 The primary source in vivo of TNF- is thought to be the monocyte/macrophage but various cell types are known to express this cytokine such as lymphocytes, basophils, eosinophils, mast cells, NK cells, T cells, B cells, kerantinocytes, Kupffer cells, astrocytes, and some types of tumours. TNF- is produced upon stimulation with cytokines such as IL-1, 1L-2, GM-CSF, TNF- itself and with bacterial lipopolysaccharide (LPS) which is a potent inducer.4 TNF- once produced and secreted will bind to TNF- receptors (TNF- R1, 55 kDa and TNF- R2, 75 kDa), located on the plasma membrane of most cells throughout the body except the red blood cell.5 The two TNF- receptors deliberate the biological effects of TNF- . It has been reported that TNF- R1 is responsible for mediating LPS toxicity6 and cell cytotoxicity7 and TNF- R2 is involved in cellular proliferation.8 Various pathological conditions are associated with the production of high levels of TNF- . These include septic shock syndrome, cachexia (e.g. HIV, tuberculosis, cancer), autoimmune diseases, hepatitis, leukemia, myocardial ischaemia, organ transplantation rejection, multiple sclerosis, rheumatoid arthritis, and meningococcal septicemia.4,9,1,11 Many people die annually from a complication of infectious disease called septic shock syndrome which is triggered by TNF- . In many cases elevated TNF- serum levels predict a higher mortality.12 TNF- is a pleiotropic cytokine that can induce disease through TNF- toxicity (tissue injury, catabolic illness, and mediating shock) and improve host defense mechanisms (stimulating inflammation and increasing immune cell function).4 In the future, therapies may be developed by blocking TNF- harmful effects and enhancing TNF- beneficial effects. This TNF- ELISA is a 2.5-3 hour solid phase immunoassay readily applicable to measure TNF- levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 1 pg/mL. It showed no cross reactivity with other cytokines tested. This TNF- ELISA is expected to be effectively used for further investigations into the relationship between TNF- and the various conditions mentioned. S7.5(5) TNF-

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation