IL-2 ELISA Kit

Produktnummer ABIN411296

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus IL-2. Es wurde in 26 Publikationen zitiert.

Kurzübersicht für IL-2 ELISA Kit (ABIN411296)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Bindungsspezifität: AA 21-169

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails IL-2 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Mouse IL-2 ELISA Kit to quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A21-Q169

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5.7%, 5.5%, 7.7%
Inter-Assay Precision (CV%): 6.5%, 6.4%, 8.2%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-2 ELISA Kit (ABIN411296)

Shen, Zhang, Li, Liu, Yu, Hu, Hui, Guo, Zhang: "Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer." in: Oncotarget, Vol. 8, Issue 30, pp. 49395-49408, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Liang, Mao, Sun, Li, Li, Yu, Ma, Gu, Zhang, Taniguchi, Li: "Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation." in: Frontiers in immunology, Vol. 9, pp. 78, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Wang, Liu, Chen, Chen: "Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 160-170, (2017) (PubMed).

Yang, Shi, Wumaierniyazi, Shan, Miao, Wu, Zhou, Yusup, Upur, Aikemu: "Enhanced antitumor efficacy and reduced toxicity of Abnormal Savda Munziq on tumor bearing mice treated with chemotherapy." in: Oncotarget, Vol. 8, Issue 54, pp. 92682-92698, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Chen, Zhang, Sun: "Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica." in: BMC complementary and alternative medicine, Vol. 15, Issue 1, pp. 427, (2016) (PubMed).

Huai, Dong, Zhu, Li, Cao, Gao, Yang, Wang, Mao: "Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection." in: Advanced healthcare materials, Vol. 5, Issue 7, pp. 786-94, (2016) (PubMed).

Xie, Cao, Zhao, Yu, Qi, Dai: "IDO expressing dendritic cells suppress allograft rejection of small bowel transplantation in mice by expansion of Foxp3+ regulatory T cells." in: Transplant immunology, Vol. 33, Issue 2, pp. 69-77, (2015) (PubMed).

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." in: BMC complementary and alternative medicine, Vol. 15, pp. 119, (2015) (PubMed).

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Eker, Yavasci, Cangur, Kirli, Sarandol: "Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?" in: Acta neuropsychiatrica, Vol. 26, Issue 5, pp. 291-7, (2014) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Meng, Ning, Qi, He, Jie, Lin, Huang, Li, Li: "Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp." in: International journal of molecular sciences, Vol. 15, Issue 3, pp. 5140-62, (2014) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Zhong, Cheng, Long, He, Qi, Xiang, Zhao, Zhu: "Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma." in: Journal of translational medicine, Vol. 11, pp. 71, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Chen, Ni, Ye, Sun, Li, Xu: "Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo." in: European journal of pharmacology, Vol. 685, Issue 1-3, pp. 186-97, (2012) (PubMed).

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: IL2

Hintergrund:

Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

Gene Full Name: interleukin 2

Gen-ID: 16183

UniProt: P04351

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation