ZAP70 FLISA Kit

Produktnummer ABIN5691979

Dieses Fluorometric ELISA kit dient der quantitativen Messung von Human und Maus ZAP70.

Kurzübersicht für ZAP70 FLISA Kit (ABIN5691979)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Bindungsspezifität: pTyr315

Reaktivität: Human, Maus

Nachweismethode: Fluorometric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails ZAP70 FLISA Kit

Verwendungszweck: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Analytische Methode: Qualitative

Produktmerkmale: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Bestandteile: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Anwendungsinformationen

Applikationshinweise: Detection Range: At least 5000 cells/well

Testdauer: 4.5 h

Plattentyp: Uncoated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C/6 Months

Haltbarkeit: 6 months

Antigendetails

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP-70

Gen-ID: 7535

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway