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ZAP70 ELISA Kit

ZAP70 Reaktivität: Human pTyr493 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN2747919
  • Target Alle ZAP70 ELISA Kits anzeigen
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Bindungsspezifität
    pTyr493
    Reaktivität
    • 9
    • 5
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-ZAP70 (Y493) ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle ZAP70 ELISA Kits anzeigen
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Andere Bezeichnung
    ZAP70 (ZAP70 Produkte)
    Synonyme
    SRK ELISA Kit, STCD ELISA Kit, STD ELISA Kit, TZK ELISA Kit, ZAP-70 ELISA Kit, 70kDa ELISA Kit, AI327364 ELISA Kit, Srk ELISA Kit, mrtle ELISA Kit, mur ELISA Kit, zap70 ELISA Kit, MGC80670 ELISA Kit, MGC88962 ELISA Kit, ZAP70 ELISA Kit, zgc:110383 ELISA Kit, zeta chain of T-cell receptor associated protein kinase 70 ELISA Kit, putative tyrosine-protein kinase zap-70 ELISA Kit, zeta-chain (TCR) associated protein kinase ELISA Kit, zeta chain of T cell receptor associated protein kinase 70 ELISA Kit, zeta-chain (TCR) associated protein kinase 70kDa ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa ELISA Kit, tyrosine-protein kinase ZAP-70 ELISA Kit, ZAP70 ELISA Kit, Smp_149470 ELISA Kit, Zap70 ELISA Kit, zap70.L ELISA Kit, zap70 ELISA Kit, LOC707555 ELISA Kit, LOC100342021 ELISA Kit, LOC100390922 ELISA Kit, LOC100542877 ELISA Kit
    Hintergrund
    Zeta-Chain Associated Protein Kinase 70 (ZAP70) phosphorylated at Tyrosine-493
    Gen-ID
    22637
    UniProt
    P43403
    Pathways
    T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
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