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CD44 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CD44 in Cell Culture Supernatant, Cell Samples, Plasma, Serum und Tissue Lysate.
Produktnummer ABIN5690845

Kurzübersicht für CD44 ELISA Kit (ABIN5690845)

Target

Alle CD44 ELISA Kits anzeigen
CD44

Reaktivität

  • 4
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • Verwendungszweck

    Human CD44/Heparan Sulfate Proteoglycan ELISA Kit.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA antibody pair recognizes Human CD44.

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C

    Haltbarkeit

    6 months
  • Target Alle CD44 ELISA Kits anzeigen

    CD44

    Andere Bezeichnung

    CD44 (Heparan Sulfate Proteoglycan

    Gen-ID

    960

    UniProt

    P16070

    Pathways

    Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling
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