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CD44 ELISA Kit

CD44 Reaktivität: Ratte AA 22-271 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN2859263
  • Target Alle CD44 ELISA Kits anzeigen
    CD44
    Bindungsspezifität
    AA 22-271
    Reaktivität
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312-20.000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD44
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: Q22-E271
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q22-E271
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 1 Link domain.

    Plattentyp
    Pre-coated
    Protokoll
    rat CD44 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD44 has been precoated onto 96-well plates. Standards(NSO, Q22 - E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD44 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL rat CD44 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CD44 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 1.76, Standard deviation: 0.09, CV(%): 5.1
    • Sample 2: n=16, Mean(ng/ml): 5.5, Standard deviation: 0.35, CV(%): 6.4
    • Sample 3: n=16, Mean(ng/ml): 12.8, Standard deviation: 0.73, CV(%): 5.7,
    • Sample 1: n=24, Mean(ng/ml): 2.1, Standard deviation: 0.16, CV(%): 7.8
    • Sample 2: n=24, Mean(ng/ml): 7.6, Standard deviation: 0.52, CV(%): 6.9
    • Sample 3: n=24, Mean(ng/ml): 14, Standard deviation: 0.88, CV(%): 6.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle CD44 ELISA Kits anzeigen
    CD44
    Andere Bezeichnung
    CD44 (CD44 Produkte)
    Synonyme
    CDW44 ELISA Kit, CSPG8 ELISA Kit, ECMR-III ELISA Kit, HCELL ELISA Kit, HUTCH-I ELISA Kit, IN ELISA Kit, LHR ELISA Kit, MC56 ELISA Kit, MDU2 ELISA Kit, MDU3 ELISA Kit, MIC4 ELISA Kit, Pgp1 ELISA Kit, AU023126 ELISA Kit, AW121933 ELISA Kit, AW146109 ELISA Kit, HERMES ELISA Kit, Ly-24 ELISA Kit, Pgp-1 ELISA Kit, CD44A ELISA Kit, METAA ELISA Kit, RHAMM ELISA Kit, XCD44 ELISA Kit, cd44 ELISA Kit, cdw44 ELISA Kit, cspg8 ELISA Kit, ecmr-iii ELISA Kit, hcell ELISA Kit, lhr ELISA Kit, mc56 ELISA Kit, mdu2 ELISA Kit, mdu3 ELISA Kit, mic4 ELISA Kit, mutch-i ELISA Kit, pgp1 ELISA Kit, CD44 molecule (Indian blood group) ELISA Kit, syndecan 4 ELISA Kit, CD44 antigen ELISA Kit, CD44 molecule (Indian blood group) S homeolog ELISA Kit, CD44 ELISA Kit, SDC4 ELISA Kit, Cd44 ELISA Kit, cd44.S ELISA Kit
    Hintergrund

    Protein Function: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9, the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function. .

    Background: CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

    Synonyms: CD44 antigen,Extracellular matrix receptor III,ECMR-III,GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD44,Cd44,

    Full Gene Name: CD44 antigen

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Colocalizes with actin in membrane protrusions at wounding edges..
    Gen-ID
    25406
    UniProt
    P26051
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling
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