CD40 Ligand ELISA Kit

Produktnummer ABIN5690771

Dieses Hund CD40 Ligand ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Hund CD40 Ligand zu quantifizieren.

Kurzübersicht für CD40 Ligand ELISA Kit (ABIN5690771)

Target: CD40 Ligand (CD40LG)

Reaktivität: Hund

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails CD40 Ligand ELISA Kit

Verwendungszweck: Canine (dog) CD40 Ligand/TNFSF5 ELISA Kit.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Canine (dog) CD40 Ligand.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu CD40 Ligand

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD40 Ligand (TNFSF5

Gen-ID: 403468

UniProt: O97626

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints