WISP1 ELISA Kit

Produktnummer ABIN5660036

Produktdetails WISP1 ELISA Kit

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6.25 pg/mL - 400 pg/mL

Untere Nachweisgrenze: 6.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of WNT1 Inducible Signaling Pathway Protein 1 (WISP1). No significant cross-reactivity or interference between WNT1 Inducible Signaling Pathway Protein 1 (WISP1) and analogues was observed.

Sensitivität: 2.55 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Andere Bezeichnung: WNT1 Inducible Signaling Pathway Protein 1 (WISP1 Produkte)

Synonyme: CCN4 ELISA Kit, WISP1c ELISA Kit, WISP1i ELISA Kit, WISP1tc ELISA Kit, WISP-1 ELISA Kit, WISP1 ELISA Kit, si:ch211-38f10.2 ELISA Kit, AW146261 ELISA Kit, Elm1 ELISA Kit, zgc:172276 ELISA Kit, WNT1 inducible signaling pathway protein 1 ELISA Kit, WNT1 inducible signaling pathway protein 1a ELISA Kit, WNT1-inducible-signaling pathway protein 1-like ELISA Kit, WNT1 inducible signaling pathway protein 1b ELISA Kit, WISP1 ELISA Kit, wisp1a ELISA Kit, Wisp1 ELISA Kit, LOC100627044 ELISA Kit, wisp1b ELISA Kit

Hintergrund:

Gene Name: WNT1 Inducible Signaling Pathway Protein 1

Gene Aliases: CCN4, WISP1c, WISP1i, WISP1tc, CCN family member 4, Wnt-1-induced secreted protein

Gen-ID: 22402

UniProt: O54775

Pathways: WNT Signalweg, Growth Factor Binding