PKC zeta ELISA Kit
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- Target Alle PKC zeta (PRKCZ) ELISA Kits anzeigen
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Protein Kinase C Zeta (PKCz). No significant cross-reactivity or interference between Protein Kinase C Zeta (PKCz) and analogues was observed.
- Sensitivität
- 0.125 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Zeta (PKCz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Zeta (PKCz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Zeta (PKCz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Zeta (PKCz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Zeta (PKCz) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Zeta (PKCz) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle PKC zeta (PRKCZ) ELISA Kits anzeigen
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
- Andere Bezeichnung
- Protein Kinase C Zeta (PRKCZ Produkte)
- Synonyme
- TPKC ELISA Kit, PKCzeta ELISA Kit, prkcz-A ELISA Kit, pkc-zeta ELISA Kit, PRKCZ ELISA Kit, pkc2 ELISA Kit, PKC-ZETA ELISA Kit, PKC2 ELISA Kit, AI098070 ELISA Kit, C80388 ELISA Kit, Pkcz ELISA Kit, R74924 ELISA Kit, aPKCzeta ELISA Kit, zetaPKC ELISA Kit, 14-3-3-zetaisoform ELISA Kit, r14-3-3 ELISA Kit, pkcz ELISA Kit, si:ch211-150o23.4 ELISA Kit, protein kinase C zeta L homeolog ELISA Kit, protein kinase C zeta ELISA Kit, protein kinase C, zeta ELISA Kit, prkcz.L ELISA Kit, PRKCZ ELISA Kit, prkcz ELISA Kit, Prkcz ELISA Kit
- Hintergrund
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Gene Name: Protein Kinase C Zeta
Gene Aliases: PKC2, PKC-Z, PRKCZ, Protein Kinase M Zeta
- Gen-ID
- 5590
- UniProt
- Q05513
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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