PARP1 ELISA Kit

Produktnummer ABIN5657974

Das Human PARP1 ELISA Kit (ABIN5657974) ist ein Colorimetric ELISA Kit zur Detektion von Human PARP1.

Kurzübersicht für PARP1 ELISA Kit (ABIN5657974)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails PARP1 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Verwendungszweck: Human Poly ADP Ribose Polymerase (PARP) ELISA Kit for detecting PARP in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.

Sensitivität: 0.28 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly ADP Ribose Polymerase (PARP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poly ADP Ribose Polymerase (PARP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: Poly ADP Ribose Polymerase

Hintergrund: Gene Aliases: PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD (+) ADP-ribosyltransferase 1

Gen-ID: 142

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location