SMAD4 ELISA Kit

Produktnummer ABIN5656968

Produktdetails SMAD4 ELISA Kit

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 4 (Smad4). No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 4 (Smad4) and analogues was observed.

Sensitivität: 0.113 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 4 (Smad4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 4 (Smad4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 4 (Smad4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 4 (Smad4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 4 (Smad4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 4 (Smad4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: Mothers Against Decapentaplegic Homolog 4 (SMAD4 Produkte)

Synonyme: smad4 ELISA Kit, DPC4 ELISA Kit, JIP ELISA Kit, MADH4 ELISA Kit, MYHRS ELISA Kit, AW743858 ELISA Kit, D18Wsu70e ELISA Kit, Madh4 ELISA Kit, XSmad4alpha ELISA Kit, Xsmad4 ELISA Kit, dpc4 ELISA Kit, jip ELISA Kit, madh4 ELISA Kit, xsmad4a ELISA Kit, SMAD family member 4 ELISA Kit, Smad4 protein ELISA Kit, SMAD family member 4, gene 1 L homeolog ELISA Kit, SMAD4 ELISA Kit, smad4 ELISA Kit, Smad4 ELISA Kit, smad4.1.L ELISA Kit

Hintergrund:

Gene Name: Mothers Against Decapentaplegic Homolog 4

Gene Aliases: DPC4, JIP, MADH4, Deletion target in pancreatic carcinoma 4

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie