MAPK13 ELISA Kit

Produktnummer ABIN5656923

Produktdetails MAPK13 ELISA Kit

Target: MAPK13 (Mitogen-Activated Protein Kinase 13 (MAPK13))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase 13 (MAPK13). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase 13 (MAPK13) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 13 (MAPK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 13 (MAPK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 13 (MAPK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase 13 (MAPK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 13 (MAPK13) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 13 (MAPK13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MAPK13

Target: MAPK13 (Mitogen-Activated Protein Kinase 13 (MAPK13))

Andere Bezeichnung: Mitogen Activated Protein Kinase 13 (MAPK13 Produkte)

Synonyme: F24B9.3 ELISA Kit, F24B9_3 ELISA Kit, MAPK 13 ELISA Kit, MAPK-13 ELISA Kit, PRKM13 ELISA Kit, SAPK4 ELISA Kit, p38delta ELISA Kit, Serk4 ELISA Kit, Prkm13 ELISA Kit, sapk4 ELISA Kit, prkm13 ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 13 ELISA Kit, mitogen activated protein kinase 13 ELISA Kit, ATMPK13 ELISA Kit, MAPK13 ELISA Kit, Mapk13 ELISA Kit, mapk13 ELISA Kit

Hintergrund:

Gene Name: Mitogen Activated Protein Kinase 13

Gene Aliases: SAPK4, p38 Delta, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4

Gen-ID: 5603

UniProt: O15264

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Hepatitis C, BCR Signaling, S100 Proteine