Midkine ELISA Kit

Produktnummer ABIN5656905

Produktdetails Midkine ELISA Kit

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Midkine (MK). No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midkine (MK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midkine (MK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midkine (MK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Midkine (MK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Midkine (MK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Midkine (MK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Andere Bezeichnung: Midkine (MDK Produkte)

Synonyme: ARAP ELISA Kit, MK ELISA Kit, NEGF2 ELISA Kit, Mek ELISA Kit, MK-B ELISA Kit, PTF-alpha-1 ELISA Kit, mdk ELISA Kit, midkine ELISA Kit, negf2 ELISA Kit, RIHB ELISA Kit, cb518 ELISA Kit, fb79b05 ELISA Kit, fj33b02 ELISA Kit, fj48a12 ELISA Kit, mdk2 ELISA Kit, plei2 ELISA Kit, wu:fb79b05 ELISA Kit, wu:fj33b02 ELISA Kit, wu:fj48a12 ELISA Kit, MK-A ELISA Kit, PTF-alpha-2 ELISA Kit, X-PTF-alpha1 ELISA Kit, XMK ELISA Kit, mdk-a ELISA Kit, mdk-b ELISA Kit, midkine ELISA Kit, midkine L homeolog ELISA Kit, midkine (neurite growth-promoting factor 2) ELISA Kit, midkine b ELISA Kit, midkine S homeolog ELISA Kit, MDK ELISA Kit, Mdk ELISA Kit, mdk.L ELISA Kit, mdkb ELISA Kit, mdk.S ELISA Kit

Hintergrund:

Gene Name: Midkine

Gene Aliases: MDK, ARAP, NEGF2, Neurite Growth-Promoting Factor 2, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2

Gen-ID: 17242

UniProt: P12025

Pathways: RTK Signalweg, M Phase, Skeletal Muscle Fiber Development