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IRAK2 ELISA Kit

IRAK2 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5655700
  • Target Alle IRAK2 ELISA Kits anzeigen
    IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.78 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.78 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 2 (IRAK2). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 2 (IRAK2) and analogues was observed.
    Sensitivität
    0.27 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 2 (IRAK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 2 (IRAK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle IRAK2 ELISA Kits anzeigen
    IRAK2 (Interleukin-1 Receptor-Associated Kinase 2 (IRAK2))
    Andere Bezeichnung
    Interleukin 1 Receptor Associated Kinase 2 (IRAK2 Produkte)
    Synonyme
    IRAK-2 ELISA Kit, 6330415L08Rik ELISA Kit, AI649099 ELISA Kit, interleukin 1 receptor associated kinase 2 ELISA Kit, interleukin-1 receptor-associated kinase 2 ELISA Kit, IRAK2 ELISA Kit, Irak2 ELISA Kit
    Hintergrund

    Gene Name: Interleukin 1 Receptor Associated Kinase 2

    Gen-ID
    3656
    UniProt
    O43187
    Pathways
    TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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