Insulin Receptor ELISA Kit

Produktnummer ABIN5655460

Das Human Insulin Receptor ELISA Kit (ABIN5655460) ist ein Colorimetric ELISA Kit zur Detektion von Human Insulin Receptor.

Kurzübersicht für Insulin Receptor ELISA Kit (ABIN5655460)

Target: Insulin Receptor (INSR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Insulin Receptor ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Insulin Receptor (ISR). No significant cross-reactivity or interference between Insulin Receptor (ISR) and analogues was observed.

Sensitivität: 1.37 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Insulin Receptor (ISR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Insulin Receptor (ISR) and unlabeled Insulin Receptor (ISR) (Standards or samples) with the pre-coated antibody specific to Insulin Receptor (ISR). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Receptor (ISR) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Receptor (ISR) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: Insulin Receptor

Hintergrund:

Gene Name: Insulin Receptor

Gene Aliases: CD220, INSR, HHF5, IR

Gen-ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity