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Hexokinase 2 ELISA Kit

HK2 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655114
  • Target Alle Hexokinase 2 (HK2) ELISA Kits anzeigen
    Hexokinase 2 (HK2)
    Reaktivität
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Hexokinase 2 (HK2). No significant cross-reactivity or interference between Hexokinase 2 (HK2) and analogues was observed.
    Sensitivität
    0.063 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexokinase 2 (HK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexokinase 2 (HK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexokinase 2 (HK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexokinase 2 (HK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexokinase 2 (HK2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexokinase 2 (HK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Hexokinase 2 (HK2) ELISA Kits anzeigen
    Hexokinase 2 (HK2)
    Andere Bezeichnung
    Hexokinase 2 (HK2 Produkte)
    Synonyme
    HKII ELISA Kit, HXK2 ELISA Kit, AI642394 ELISA Kit, hexokinase-2 ELISA Kit, fi09d05 ELISA Kit, fi17h06 ELISA Kit, wu:fi09d05 ELISA Kit, wu:fi17h06 ELISA Kit, wu:fi31b04 ELISA Kit, zgc:55926 ELISA Kit, HK2 ELISA Kit, ARABIDOPSIS THALIANA HEXOKINASE 2 ELISA Kit, ATHXK2 ELISA Kit, F6F22.11 ELISA Kit, F6F22_11 ELISA Kit, HEXOKINASE 2 ELISA Kit, hexokinase 2 ELISA Kit, StHK2 ELISA Kit, hexokinase 2 ELISA Kit, hexokinase-2 ELISA Kit, hexokinase 2 L homeolog ELISA Kit, HK2 ELISA Kit, Hk2 ELISA Kit, hk2 ELISA Kit, HXK2 ELISA Kit, LOC100032849 ELISA Kit, hk2.L ELISA Kit, hxk2 ELISA Kit, LOC102577690 ELISA Kit
    Hintergrund

    Gene Name: Hexokinase 2

    Gene Aliases: HK II, Muscle form hexokinase

    Gen-ID
    15277
    UniProt
    O08528
    Pathways
    PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt
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