GDNF CLIA Kit

Produktnummer ABIN5654569

Produktdetails GDNF CLIA Kit

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor (GDNF). No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor (GDNF) and analogues was observed.

Sensitivität: 3.12 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glial Cell Line Derived Neurotrophic Factor (GDNF) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: Glial Cell Line Derived Neurotrophic Factor (GDNF Produkte)

Synonyme: gdnf CLIA Kit, GDNF CLIA Kit, AI385739 CLIA Kit, gdnfa CLIA Kit, hfb1-gdnf CLIA Kit, ATF1 CLIA Kit, ATF2 CLIA Kit, HFB1-GDNF CLIA Kit, HSCR3 CLIA Kit, glial cell derived neurotrophic factor CLIA Kit, glial cell derived neurotrophic factor a CLIA Kit, glial cell line derived neurotrophic factor CLIA Kit, glial cell derived neurotrophic factor L homeolog CLIA Kit, Gdnf CLIA Kit, gdnfa CLIA Kit, GDNF CLIA Kit, gdnf.L CLIA Kit

Hintergrund:

Gene Name: Glial Cell Line Derived Neurotrophic Factor

Gene Aliases: ATF1, ATF2, HFB1-GDNF, Astrocyte-Derived Trophic Factor, Glial Derived Neurotrophic Factor

Gen-ID: 14573

UniProt: P48540

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration