GIP ELISA Kit

Produktnummer ABIN5654515

Produktdetails GIP ELISA Kit

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 61.7 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 61.7 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Gastric Inhibitory Polypeptide (GIP). No significant cross-reactivity or interference between Gastric Inhibitory Polypeptide (GIP) and analogues was observed.

Sensitivität: 23.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastric Inhibitory Polypeptide (GIP) and unlabeled Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GIP

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Andere Bezeichnung: Gastric Inhibitory Polypeptide (GIP Produkte)

Synonyme: GIP ELISA Kit, xgip ELISA Kit, Gludins ELISA Kit, RATGLUDINS ELISA Kit, gastric inhibitory polypeptide ELISA Kit, gastric inhibitory polypeptide L homeolog ELISA Kit, GIP ELISA Kit, gip ELISA Kit, Gip ELISA Kit, gip.L ELISA Kit

Hintergrund:

Gene Name: Gastric Inhibitory Polypeptide

Gene Aliases: Incretin hormone, Glucose Dependent Insulinotropic Peptide

Gen-ID: 2695

UniProt: P09681

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism