Cyclin D1 ELISA Kit

Produktnummer ABIN5653414

Produktdetails Cyclin D1 ELISA Kit

Target: Cyclin D1 (CCND1)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cyclin D1 (CCND1). No significant cross-reactivity or interference between Cyclin D1 (CCND1) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D1 (CCND1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D1 (CCND1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin D1 (CCND1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin D1 (CCND1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin D1 (CCND1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin D1 (CCND1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1 (CCND1 Produkte)

Synonyme: BCL1 ELISA Kit, D11S287E ELISA Kit, PRAD1 ELISA Kit, U21B31 ELISA Kit, AI327039 ELISA Kit, CycD1 ELISA Kit, Cyl-1 ELISA Kit, bcl-1 ELISA Kit, cD1 ELISA Kit, cb161 ELISA Kit, cycd1 ELISA Kit, etID37810.7 ELISA Kit, fb52e01 ELISA Kit, fc45c08 ELISA Kit, fc83a12 ELISA Kit, id:ibd1198 ELISA Kit, wu:fb52e01 ELISA Kit, wu:fc45c08 ELISA Kit, wu:fc83a12 ELISA Kit, ccnd1b ELISA Kit, prad1 ELISA Kit, ccnd1 ELISA Kit, ccnd1a ELISA Kit, bcl1 ELISA Kit, d11s287e ELISA Kit, u21b31 ELISA Kit, CCND1 ELISA Kit, cyclin D1 ELISA Kit, cyclin D1 S homeolog ELISA Kit, cyclin D1 L homeolog ELISA Kit, CCND1 ELISA Kit, Ccnd1 ELISA Kit, ccnd1 ELISA Kit, ccnd1.S ELISA Kit, ccnd1.L ELISA Kit

Hintergrund:

Gene Name: Cyclin D1

Gene Aliases: CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene

Gen-ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling