BDNF CLIA Kit
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- Target Alle BDNF CLIA Kits anzeigen
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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Reaktivität
- Human
- Nachweismethode
- Chemiluminescent
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 27.4 pg/mL - 20000 pg/mL
- Untere Nachweisgrenze
- 27.4 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
- Sensitivität
- 11.2 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 - 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The microplate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Brain Derived Neurotrophic Factor (BDNF) level in the sample or standard.,
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle BDNF CLIA Kits anzeigen
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Andere Bezeichnung
- Brain Derived Neurotrophic Factor (BDNF Produkte)
- Synonyme
- ANON2 CLIA Kit, BULN2 CLIA Kit, bdnf-A CLIA Kit, BDNF CLIA Kit, bdnf CLIA Kit, brain derived neurotrophic factor CLIA Kit, brain-derived neurotrophic factor CLIA Kit, brain-derived neurotrophic factor L homeolog CLIA Kit, brain-derived neurotrophic factor S homeolog CLIA Kit, BDNF CLIA Kit, Bdnf CLIA Kit, bdnf CLIA Kit, bdnf.L CLIA Kit, bdnf.S CLIA Kit
- Hintergrund
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Gene Name: Brain Derived Neurotrophic Factor
Gene Aliases: Neurotrophin, Abrineurin
- Gen-ID
- 627
- UniProt
- P23560
- Pathways
- RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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