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AIF ELISA Kit

AIFM1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5651957
  • Target Alle AIF (AIFM1) ELISA Kits anzeigen
    AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
    Reaktivität
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Apoptosis Inducing Factor (AIF). No significant cross-reactivity or interference between Apoptosis Inducing Factor (AIF) and analogues was observed.
    Sensitivität
    0.061 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inducing Factor (AIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inducing Factor (AIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Inducing Factor (AIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Inducing Factor (AIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle AIF (AIFM1) ELISA Kits anzeigen
    AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
    Andere Bezeichnung
    Apoptosis Inducing Factor (AIFM1 Produkte)
    Synonyme
    AIF ELISA Kit, CMTX4 ELISA Kit, COWCK ELISA Kit, COXPD6 ELISA Kit, PDCD8 ELISA Kit, CG7263 ELISA Kit, DmAIF ELISA Kit, Dmel\\CG7263 ELISA Kit, GB16024 ELISA Kit, DDBDRAFT_0187853 ELISA Kit, DDBDRAFT_0191137 ELISA Kit, DDB_0187853 ELISA Kit, DDB_0191137 ELISA Kit, aif ELISA Kit, pdcd8 ELISA Kit, AIFM1 ELISA Kit, PCD8 ELISA Kit, AIFsh2 ELISA Kit, Hq ELISA Kit, Pdcd8 ELISA Kit, mAIF ELISA Kit, Aif ELISA Kit, zgc:91994 ELISA Kit, apoptosis inducing factor mitochondria associated 1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, Apoptosis inducing factor ELISA Kit, apoptosis-inducing factor 1, mitochondrial ELISA Kit, apoptosis inducing factor ELISA Kit, apoptosis inducing factor, mitochondria associated 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated 1 ELISA Kit, AIFM1 ELISA Kit, AIF1 ELISA Kit, AIF ELISA Kit, LOC412212 ELISA Kit, aif ELISA Kit, aifm1 ELISA Kit, Aifm1 ELISA Kit
    Hintergrund

    Gene Name: Apoptosis Inducing Factor

    Gene Aliases: PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial

    Pathways
    Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effekt
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