Acetyl-CoA Carboxylase alpha ELISA Kit

Produktnummer ABIN5651340

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Acetyl-CoA Carboxylase alpha.

Kurzübersicht für Acetyl-CoA Carboxylase alpha ELISA Kit (ABIN5651340)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails Acetyl-CoA Carboxylase alpha ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Verwendungszweck: Human Acetyl Coenzyme A Carboxylase Alpha (ACACa) ELISA Kit for detecting ACACa in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Carboxylase Alpha (ACACa). No significant cross-reactivity or interference between Acetyl Coenzyme A Carboxylase Alpha (ACACa) and analogues was observed.

Sensitivität: 0.29 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetyl Coenzyme A Carboxylase Alpha (ACACa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetyl Coenzyme A Carboxylase Alpha (ACACa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Andere Bezeichnung: Acetyl Coenzyme A Carboxylase Alpha

Hintergrund: Gene Aliases: ACAC, ACC1, ACCA, ACAC, ACC2, ACCB, ACACB, ACACA, Acetyl CoA Carboxylase, Biotin carboxylase

Gen-ID: 31

UniProt: Q13085

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effekt