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Acetyl-CoA Carboxylase alpha ELISA Kit

ACACA Reaktivität: Human, Ratte, Maus pSer79 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4885957
  • Target Alle Acetyl-CoA Carboxylase alpha (ACACA) ELISA Kits anzeigen
    Acetyl-CoA Carboxylase alpha (ACACA)
    Bindungsspezifität
    pSer79
    Reaktivität
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Phospho-ACC1 (S79) ELISA Kit. This assay semi-quantitatively measures phophorylated ACC1 (Ser79) in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human, mouse, and rat ACC1 phosphorylated at site Serine-79.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle Acetyl-CoA Carboxylase alpha (ACACA) ELISA Kits anzeigen
    Acetyl-CoA Carboxylase alpha (ACACA)
    Andere Bezeichnung
    ACC1 (ACACA Produkte)
    Synonyme
    ACAC ELISA Kit, ACACAD ELISA Kit, ACC ELISA Kit, ACC1 ELISA Kit, ACCA ELISA Kit, A530025K05Rik ELISA Kit, Acac ELISA Kit, Acc1 ELISA Kit, Gm738 ELISA Kit, AT-ACC1 ELISA Kit, EMB22 ELISA Kit, EMBRYO DEFECTIVE 22 ELISA Kit, GK ELISA Kit, GURKE ELISA Kit, PAS3 ELISA Kit, PASTICCINO 3 ELISA Kit, acetyl-CoA carboxylase 1 ELISA Kit, acc ELISA Kit, fj43d01 ELISA Kit, im:7138837 ELISA Kit, si:ch211-199d18.1 ELISA Kit, wu:fj43d01 ELISA Kit, acetyl-CoA carboxylase alpha ELISA Kit, acetyl-Coenzyme A carboxylase alpha ELISA Kit, acetyl-CoA carboxylase 1 ELISA Kit, ACACA ELISA Kit, Acaca ELISA Kit, ACC1 ELISA Kit, acaca ELISA Kit
    Gen-ID
    31
    UniProt
    Q13085
    Pathways
    AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effekt
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