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TBK1 ELISA Kit

TBK1 Reaktivität: Human, Maus, Ratte pSer172 Colorimetric Cell ELISA Adherent Cell Culture
Produktnummer ABIN5526719
  • Target Alle TBK1 ELISA Kits anzeigen
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Bindungsspezifität
    pSer172, total
    Reaktivität
    Human, Maus, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Cell ELISA
    Applikation
    ELISA
    Verwendungszweck
    Cell-Based ELISA Kit. This assay semi-quantitatively measures TBK1 phosphorylated at Serine-172 as well as total TBK1 in adherent cell lines.
    Proben
    Adherent Cell Culture
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human, Mouse and Rat TBK1 phosphorylated at site Serine-172 as well as total TBK1.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Bestandteile
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle TBK1 ELISA Kits anzeigen
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Andere Bezeichnung
    TBK1 (TBK1 Produkte)
    Synonyme
    nak ELISA Kit, t2k ELISA Kit, TBK1 ELISA Kit, wu:fk70c05 ELISA Kit, zgc:136548 ELISA Kit, NAK ELISA Kit, T2K ELISA Kit, 1200008B05Rik ELISA Kit, AI462036 ELISA Kit, AW048562 ELISA Kit, TANK binding kinase 1 L homeolog ELISA Kit, TANK binding kinase 1 ELISA Kit, TANK-binding kinase 1 ELISA Kit, tbk1.L ELISA Kit, TBK1 ELISA Kit, tbk1 ELISA Kit, Tbk1 ELISA Kit
    Gen-ID
    29110
    UniProt
    Q9UHD2, Q9WUN2, D4A7D3
    Pathways
    TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom
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