Leptin ELISA Kit

Produktnummer ABIN5068384

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Leptin.

Kurzübersicht für Leptin ELISA Kit (ABIN5068384)

Target: Leptin (LEP)

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum

Produktdetails Leptin ELISA Kit

Sensitivität: 0.5 ng/mL

Produktmerkmale: Leptin, Serum, Human, ELISA Kit (Ob, Obese Protein, Obesity Factor)

Bestandteile:

• Microwell Strip Plate, 1x96wells
• Standard, 0ng/ml, 1x10ml
• Standard, 1ng/ml, 1x500ul
• Standard, 5ng/ml, 1x500ul
• Standard, 10ng/ml, 1x500ul
• Standard, 20ng/ml, 1x500ul
• Standard, 50ng/ml, 1x500ul
• Standard, 100ng/ml, 1x500ul
• Low Control, 1x500ul
• 0: High Control, 1x500ul
• 1: AB (Biotin), 1x10ml
• 2: Streptavidin (HRP) 50X, 1x400ul
• 3: Assay Buffer, 1x20ml
• 4: Wash Buffer, 10X, 1x50ml
• 5: TMB, 1x16ml
• 6: Stop Solution, 1x6ml

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Recently, Ob genes (mouse and human) have been cloned. Obese gene encodes an adipocyte-tissue derived secreted Ob protein/Leptin (167aa, ~16kD) that controls body weight homeostasis. Exogenous administration of recombinant Ob protein can reduce food intake and body weight. However, Ob protein had no effect in db/db mice suggesting a defect in leptin signaling mechanism. Leptin exert its effects by interacting with leptin receptors (obese receptors). The Ob-R has been shown to be a product of db gene that has long been thought to encode the receptor for a weight-controlling hormone. The Ob-R has at least 6 alternatively spliced forms with modifications at the amino and C-terminus.

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process