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Leptin ELISA Kit

Name: Leptin ELISA Kit
SKU: ABIN5068121
Price: 1129.46 EUR
Availability: InStock

Dieses Maus und Ratte Leptin ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus und Ratte Leptin zu quantifizieren.

Produktnummer ABIN5068121

Kurzübersicht für Leptin ELISA Kit (ABIN5068121)

Target

Alle Leptin (LEP) ELISA Kits anzeigen

Leptin (LEP)

Reaktivität

Maus, Ratte

Nachweismethode

Colorimetric

Applikation

ELISA

Proben

Plasma, Serum, Tissue Culture Medium

Discover our top product LEP ELISA Kit

Produktmerkmale

Leptin, Mouse and Rat ELISA Kit

Bestandteile
- Microtiter Plate 1x96 wells
- Biotin-Labeled Antibody Concentrate (10x), 1x1.3ml
- Streptavidin-HRP Conjugate, 1x13ml
- Mouse Standard 1x1vial
- Rat Standard 1x1vial
- Quality Control, Mouse 2x1vial
- Quality Control, Rat 2x1vial
- Dilution Buffer, 2x13ml
- Biotin-Antibody Diluent, 1x13ml
- 0: Wash Solution Concentrate (10x), 1x100ml
- 1: Substrate Solution (TMB), 1x13ml
- 2: Stop Solution, O4, 1x13ml
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Plattentyp

Pre-coated

Protokoll

In the Mouse and Rat Leptin ELISA, standards, quality controls, and samples are incubated in microplate wells coated with anti-mouse leptin antibody. After 60 minutes incubation and washng, biotin-labeled polyclonal anti-mouse leptin antibody is added to the wells and incubated with immobilized antibody-leptin complex for 60 minutes. After another washing, Streptavidin-HRP conjugate is added. Ater 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by the addition of acidic solution and the absorbance of the resulting yellow product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of leptin. A standard curve is constructed by plotting the absorbance values versus the leptin concentrations of standards and concentrations of unknown samples are determined using this standard curve.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Lagerung

4 °C,-20 °C

Informationen zur Lagerung

4°C/-20°C
Target Alle Leptin (LEP) ELISA Kits anzeigen

Leptin (LEP)

Andere Bezeichnung

Leptin

Pathways

JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process