IL-8 ELISA Kit

Produktnummer ABIN5026945

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human IL-8.

Kurzübersicht für IL-8 ELISA Kit (ABIN5026945)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-8 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: This assay employs an antibody specific for Anti-human IL-8 coated on a 96-well plate.

Analytische Methode: Quantitative

Sensitivität: 18.75 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma. Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 18.75 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Andere Bezeichnung: IL-8 (Interleukin 8)

Pathways: TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie