IL-27 ELISA Kit

Produktnummer ABIN5026935

Human IL-27 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human IL-27.

Kurzübersicht für IL-27 ELISA Kit (ABIN5026935)

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-27 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-27 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 9.4 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-humanIL-27 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Andere Bezeichnung: IL-27 (Interleukin 27)