IL-27 ELISA Kit

Produktnummer ABIN6720110

Produktdetails IL-27 ELISA Kit

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: IL 27A, IL 30, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27A_HUMAN, IL27p28, IL30, Interleukin 27, Interleukin 27 subunit alpha, Interleukin 30, Interleukin-27 subunit alpha, p28

Background: Interleukin-27(IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus(EBV)-induced gene 3(EBI3)(also known as IL-27B) and IL27-p28(known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.

Gene Name: IL27

Production: Natural and recombinant human IL-27. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: EBI3(R21-K229)+P28(F29-P243)

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-27 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Andere Bezeichnung: IL-27 (IL27 Produkte)

Synonyme: IL-27 ELISA Kit, IL-27A ELISA Kit, IL27A ELISA Kit, IL27p28 ELISA Kit, IL30 ELISA Kit, p28 ELISA Kit, RGD1561420 ELISA Kit, IL27 ELISA Kit, IL-27p28 ELISA Kit, Il30 ELISA Kit, interleukin 27 ELISA Kit, IL27 ELISA Kit, Il27 ELISA Kit

Gen-ID: 246778

NCBI Accession: NP_663634

UniProt: Q8NEV9