IL-11 ELISA Kit

Produktnummer ABIN5026925

Dieses Human IL-11 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-11 zu quantifizieren.

Kurzübersicht für IL-11 ELISA Kit (ABIN5026925)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-11 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-11 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: 18.7 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-11antibody was pre-coated into 96-well plates. Biotin conjugated anti-humanIL-11detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-11amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-11 can be calculated using the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Andere Bezeichnung: IL-11 (Interleukin 11)

Pathways: JAK-STAT Signalweg, Negative Regulation of Hormone Secretion