IL-11 ELISA Kit

Produktnummer ABIN6719750

Produktdetails IL-11 ELISA Kit

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Adipogenesis inhibitory factor, AGIF, IL 11, IL-11, Il11, IL11_HUMAN, Interleukin 11, Interleukin-11, Interleukin11, Oprelvekin

Background: Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.

Gene Name: IL11

Production: Natural and recombinant human IL-11. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: P22-L199

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human IL-11 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Other Performance Data: DOE: 2-8° C- 02/30/19 -20° C - 08/30/19

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Andere Bezeichnung: IL-11 (IL11 Produkte)

Synonyme: IL11 ELISA Kit, AGIF ELISA Kit, IL-11 ELISA Kit, Il-11 ELISA Kit, interleukin 11 ELISA Kit, IL11 ELISA Kit, Il11 ELISA Kit

Gen-ID: 3589

NCBI Accession: NP_000632

UniProt: P20809

Pathways: JAK-STAT Signalweg, Negative Regulation of Hormone Secretion