EGF ELISA Kit

Produktnummer ABIN5026920

Human EGF ELISA Kit, Colorimetric Assay für die Quantifizierung von Human EGF.

Kurzübersicht für EGF ELISA Kit (ABIN5026920)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9-250 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails EGF ELISA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 2.34 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human EGF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: <2.34 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human EGF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human EGF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human EGF can be calculated using the standard curve.  

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Please refer to the Manual

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF (Epidermal Growth Factor)

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation