EGF ELISA Kit
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- Target Alle EGF ELISA Kits anzeigen
- EGF (Epidermal Growth Factor (EGF))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 3.9-250 pg/mL
- Untere Nachweisgrenze
- 3.9 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- This immunoassay kit allows for the in vitro quantitative determination of Human EGF concentrations in serum, plasma and other biological fluids.
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Sensitivität
- < 2.34 pg/mL
- Bestandteile
- plate, standard, diluent, antibodies
- Benötigtes Material
- pipettes, tubes, reader, incubator
- Featured
- Zu unserem meistverkauften EGF ELISA Kit
- Top Product
- Discover our top product EGF ELISA Kit
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- Probenmenge
- 100 μL
- Testdauer
- 3 - 4 h
- Plattentyp
- Pre-coated
- Protokoll
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human EGF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human EGF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human EGF can be calculated using the standard curve.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Vorsichtsmaßnahmen
- protect your eyes
- Lagerung
- 4 °C
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- Target Alle EGF ELISA Kits anzeigen
- EGF (Epidermal Growth Factor (EGF))
- Andere Bezeichnung
- EGF (Epidermal Growth Factor) (EGF Produkte)
- Synonyme
- HOMG4 ELISA Kit, URG ELISA Kit, AI790464 ELISA Kit, CEGF ELISA Kit, epidermal growth factor ELISA Kit, pro-epidermal growth factor ELISA Kit, EGF ELISA Kit, egf ELISA Kit, CpipJ_CPIJ020278 ELISA Kit, Egf ELISA Kit
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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