IL-24 ELISA Kit

Produktnummer ABIN4986950

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-24 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) und Plasma (EDTA).

Kurzübersicht für IL-24 ELISA Kit (ABIN4986950)

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails IL-24 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human IL-24/MDA-7 Ligand

Sensitivität: 30 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-24

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Andere Bezeichnung: IL-24/MDA-7

Hintergrund: Interleukin 24(IL-24),also known as mda7 (melanoma differentiation associated gene7),is a member of the IL10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential Nlinked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 3540 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69 % aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-γ, IL-1β,IL-12, and TNF-α. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL-24 also has antiangiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20Rα and IL-20Rβ, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22R and IL-20Rβ, which is shared with IL-20.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagie