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STAT5A ELISA Kit

STAT5A Reaktivität: Human pTyr694 Colorimetric Sandwich ELISA Cell Culture Lysate
Produktnummer ABIN4889792
  • Target Alle STAT5A ELISA Kits anzeigen
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Bindungsspezifität
    pTyr694
    Reaktivität
    • 8
    • 7
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-STAT5A (Tyr694) ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 in cell lysate samples.
    Proben
    Cell Culture Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle STAT5A ELISA Kits anzeigen
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Andere Bezeichnung
    STAT5A (STAT5A Produkte)
    Synonyme
    MGF ELISA Kit, STAT5 ELISA Kit, AA959963 ELISA Kit, Stat5 ELISA Kit, STAT5A/MGF ELISA Kit, STATA5 ELISA Kit, STAT5A ELISA Kit, stat5 ELISA Kit, stat5b ELISA Kit, signal transducer and activator of transcription 5A ELISA Kit, signal transducer and activator of transcription 5a ELISA Kit, STAT5A ELISA Kit, Stat5a ELISA Kit, stat5a ELISA Kit, LOC100348518 ELISA Kit, LOC100716811 ELISA Kit
    Gen-ID
    6776
    UniProt
    P42229
    Pathways
    JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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