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CHEK2 ELISA Kit

CHEK2 Reaktivität: Human pThr68 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886029
  • Target Alle CHEK2 ELISA Kits anzeigen
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Bindungsspezifität
    pThr68, total
    Reaktivität
    • 6
    • 4
    • 3
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-CHK2 (T68) and Total CHK2 ELISA Kit. This assay semi-quantitatively measures phophorylated CHK2 (Thr68) and Total CHK2 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human CHK2 phosphorylated at site Threonine-68 and Total CHK2.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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    Discover our top product CHEK2 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle CHEK2 ELISA Kits anzeigen
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Andere Bezeichnung
    CHK2 (CHEK2 Produkte)
    Synonyme
    CDS1 ELISA Kit, CHK2 ELISA Kit, HuCds1 ELISA Kit, LFS2 ELISA Kit, PP1425 ELISA Kit, RAD53 ELISA Kit, hCds1 ELISA Kit, fa66f08 ELISA Kit, wu:fa66f08 ELISA Kit, zgc:55865 ELISA Kit, Cds1 ELISA Kit, HUCDS1 ELISA Kit, Rad53 ELISA Kit, Chk2 ELISA Kit, cds1 ELISA Kit, chek2 ELISA Kit, chk2 ELISA Kit, hucds1 ELISA Kit, lfs2 ELISA Kit, pp1425 ELISA Kit, rad53 ELISA Kit, checkpoint kinase 2 ELISA Kit, serine/threonine-protein kinase chk2 ELISA Kit, checkpoint kinase 2 L homeolog ELISA Kit, CHEK2 ELISA Kit, chek2 ELISA Kit, Chek2 ELISA Kit, MCYG_07308 ELISA Kit, chek2.L ELISA Kit
    Gen-ID
    11200
    UniProt
    O96017
    Pathways
    p53 Signalweg, Apoptose, Zellzyklus
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