PLA2G4A ELISA Kit

Produktnummer ABIN4884173

Produktdetails PLA2G4A ELISA Kit

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human PLA2G4A ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Andere Bezeichnung: PLA2G4A (PLA2G4A Produkte)

Synonyme: PLA2G4 ELISA Kit, cPLA2-alpha ELISA Kit, Pla2c ELISA Kit, Pla2g4 ELISA Kit, cPLA2 ELISA Kit, pla2g4 ELISA Kit, CPLA2 ELISA Kit, PLA2G4A ELISA Kit, PLA2 ELISA Kit, PLA2G1B ELISA Kit, cPLA2alpha ELISA Kit, pla2g4a ELISA Kit, si:dkey-97o5.1 ELISA Kit, phospholipase A2 group IVA ELISA Kit, phospholipase A2 group IVA L homeolog ELISA Kit, phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit, phospholipase A2, group IVAb (cytosolic, calcium-dependent) ELISA Kit, PLA2G4A ELISA Kit, Pla2g4a ELISA Kit, pla2g4a.L ELISA Kit, pla2g4ab ELISA Kit

Gen-ID: 5321

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling