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LIFR ELISA Kit
LIFR
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883608
Produktdetails LIFR ELISA Kit
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Target
Alle LIFR ELISA Kits anzeigen
LIFR
(Leukemia Inhibitory Factor Receptor alpha (LIFR))
Reaktivität
Alle Reaktivitäten für LIFR ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human LIF R alpha ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes human LIF R alpha. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product LIFR ELISA Kit
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LIFR
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Target
Alle LIFR ELISA Kits anzeigen
LIFR
(Leukemia Inhibitory Factor Receptor alpha (LIFR))
Andere Bezeichnung
LIF R alpha (LIFR Produkte )
Synonyme
A230075M04Rik ELISA Kit, AW061234 ELISA Kit, LIF ELISA Kit, CD118 ELISA Kit, LIF-R ELISA Kit, SJS2 ELISA Kit, STWS ELISA Kit, SWS ELISA Kit, leukemia inhibitory factor receptor ELISA Kit, LIF receptor alpha ELISA Kit, leukemia inhibitory factor receptor alpha ELISA Kit, Lifr ELISA Kit, LOC397451 ELISA Kit, LIFR ELISA Kit
Gen-ID
3977
UniProt
P42702
Pathways
JAK-STAT Signalweg , Growth Factor Binding
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