AIF ELISA Kit

Produktnummer ABIN4881777

Produktdetails AIF ELISA Kit

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human AIF ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human B7-H4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Andere Bezeichnung: AIF (AIFM1 Produkte)

Synonyme: AIF ELISA Kit, CMTX4 ELISA Kit, COWCK ELISA Kit, COXPD6 ELISA Kit, PDCD8 ELISA Kit, CG7263 ELISA Kit, DmAIF ELISA Kit, Dmel\\CG7263 ELISA Kit, GB16024 ELISA Kit, DDBDRAFT_0187853 ELISA Kit, DDBDRAFT_0191137 ELISA Kit, DDB_0187853 ELISA Kit, DDB_0191137 ELISA Kit, aif ELISA Kit, pdcd8 ELISA Kit, AIFM1 ELISA Kit, PCD8 ELISA Kit, AIFsh2 ELISA Kit, Hq ELISA Kit, Pdcd8 ELISA Kit, mAIF ELISA Kit, Aif ELISA Kit, zgc:91994 ELISA Kit, apoptosis inducing factor mitochondria associated 1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, Apoptosis inducing factor ELISA Kit, apoptosis-inducing factor 1, mitochondrial ELISA Kit, apoptosis inducing factor ELISA Kit, apoptosis inducing factor, mitochondria associated 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated 1 ELISA Kit, AIFM1 ELISA Kit, AIF1 ELISA Kit, AIF ELISA Kit, LOC412212 ELISA Kit, aif ELISA Kit, aifm1 ELISA Kit, Aifm1 ELISA Kit

Gen-ID: 9131

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effekt