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ADAM12 ELISA Kit

ADAM12 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881739
  • Target Alle ADAM12 ELISA Kits anzeigen
    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    Reaktivität
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human ADAM Metallopeptidase Domain 12 (ADAM12) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human ADAM Metallopeptidase Domain 12 (ADAM12)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ADAM12 ELISA Kits anzeigen
    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    Andere Bezeichnung
    ADAM-12 (ADAM12 Produkte)
    Synonyme
    ADAM12 ELISA Kit, adam12 ELISA Kit, Mltna ELISA Kit, mKIAA4001 ELISA Kit, MCMP ELISA Kit, MCMPMltna ELISA Kit, MLTN ELISA Kit, MLTNA ELISA Kit, ADAM metallopeptidase domain 12 ELISA Kit, a disintegrin and metallopeptidase domain 12 (meltrin alpha) ELISA Kit, ADAM12 ELISA Kit, adam12 ELISA Kit, Adam12 ELISA Kit
    Gen-ID
    8038
    UniProt
    O43184
    Pathways
    EGFR Signaling Pathway
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