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ADAM12 ELISA Kit (ADAM Metallopeptidase Domain 12) ELISA Kit

ADAM12 Reaktivität: Ratte AA 29-513 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Produktnummer ABIN1889433
$369.00
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 4 bis 6 Werktagen
  • Target
    ADAM12
    Bindungsspezifität
    AA 29-513
    Reaktivität
    Ratte
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10.000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat ADAM12
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: CHO
    Immunogen sequence: R29-S513
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: CHO
    Immunogen sequence: R29-S513
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 1 disintegrin domain.

    Plattentyp
    Pre-coated
    Protokoll
    rat ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ADAM12 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat ADAM12 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 1.46, Standard deviation: 0.066, CV(%): 4.5
    • Sample 2: n=16, Mean(ng/ml): 3.65, Standard deviation: 0.139, CV(%): 3.8
    • Sample 3: n=16, Mean(ng/ml): 6.89, Standard deviation: 0.234, CV(%): 3.4,
    • Sample 1: n=24, Mean(ng/ml): 1.55, Standard deviation: 0.088, CV(%): 5.7
    • Sample 2: n=24, Mean(ng/ml): 3.81, Standard deviation: 0.175, CV(%): 4.6
    • Sample 3: n=24, Mean(ng/ml): 6.92, Standard deviation: 0.324, CV(%): 4.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target
    ADAM12
    Andere Bezeichnung
    ADAM12 (ADAM12 ELISA Kit Abstract)
    Synonyme
    ADAM12, adam12, Mltna, mKIAA4001, MCMP, MCMPMltna, MLTN, MLTNA, ADAM metallopeptidase domain 12, a disintegrin and metallopeptidase domain 12 (meltrin alpha), ADAM12, adam12, Adam12
    Hintergrund

    Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

    Synonyms: Protein Adam12 ,Adam12 ,

    Full Gene Name: ADAM metallopeptidase domain 12

    Gen-ID
    679837
    UniProt
    F1M9Z9
    Pathways
    EGFR Signaling Pathway
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