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ADAM12 ELISA Kit

ADAM12 Reaktivität: Maus Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Produktnummer ABIN6720076
  • Target Alle ADAM12 ELISA Kits anzeigen
    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    Reaktivität
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA

    Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

    Gene Name: ADAM12

    Production: Natural and recombinant mouse ADAM12. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: CHO, Immunogen sequence: R29-S513

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  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. It is recommended that each mouse ADAM12 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10,000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle ADAM12 ELISA Kits anzeigen
    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    Andere Bezeichnung
    ADAM12 (ADAM12 Produkte)
    Synonyme
    ADAM12 ELISA Kit, adam12 ELISA Kit, Mltna ELISA Kit, mKIAA4001 ELISA Kit, MCMP ELISA Kit, MCMPMltna ELISA Kit, MLTN ELISA Kit, MLTNA ELISA Kit, ADAM metallopeptidase domain 12 ELISA Kit, a disintegrin and metallopeptidase domain 12 (meltrin alpha) ELISA Kit, ADAM12 ELISA Kit, adam12 ELISA Kit, Adam12 ELISA Kit
    Gen-ID
    11489
    NCBI Accession
    NP_031426
    UniProt
    Q61824
    Pathways
    EGFR Signaling Pathway
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