Fibronectin 1 ELISA Kit

Produktnummer ABIN455123

Produktdetails Fibronectin 1 ELISA Kit

Target: Fibronectin 1 (FN1)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of mouse Fibronectin,FN concentrations in cell culture supernates, serum, plasma and other biological fluids.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural mouse FN.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: < 11.5 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Mus musculus,Mouse,Fibronectin,FN,Fn1

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu Fibronectin 1

Target: Fibronectin 1 (FN1)

Andere Bezeichnung: Fn1 (FN1 Produkte)

Synonyme: FN1 ELISA Kit, FN ELISA Kit, cig ELISA Kit, fibronectin ELISA Kit, finc ELISA Kit, lets ELISA Kit, msf ELISA Kit, Fn ELISA Kit, fn2 ELISA Kit, fb80d10 ELISA Kit, wu:fb80d10 ELISA Kit, CIG ELISA Kit, ED-B ELISA Kit, FINC ELISA Kit, FNZ ELISA Kit, GFND ELISA Kit, GFND2 ELISA Kit, LETS ELISA Kit, MSF ELISA Kit, E330027I09 ELISA Kit, Fn-1 ELISA Kit, FIBNEC ELISA Kit, fn-1 ELISA Kit, fibronectin 1 ELISA Kit, fibronectin 1a ELISA Kit, fibronectin 1 S homeolog ELISA Kit, FN1 ELISA Kit, fn1 ELISA Kit, fn1a ELISA Kit, Fn1 ELISA Kit, fn1.S ELISA Kit

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie