Fibronectin 1 ELISA Kit

Produktnummer ABIN411271

Human Fibronectin 1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Fibronectin 1 und es wurde in 13+ Publikationen zitiert.

Kurzübersicht für Fibronectin 1 ELISA Kit (ABIN411271)

Target: Fibronectin 1 (FN1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails Fibronectin 1 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Human Fibronectin ELISA Kit to quantitate Human FN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytische Methode: Quantitative

Spezifität: Target protein isolated from human plasma

Capture antibody and Detection antibody: polyclonal antibody from rabbit, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.6%, 5.7%, 5.7%
Inter-Assay Precision (CV%): 5.7%, 6.9%, 7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Fibronectin 1 ELISA Kit (ABIN411271)

Stevens, Scull, Ramanan, Fortin, Chaturvedi, Knouse, Xiao, Fung, Mirabella, Chen, McCue, Yang, Fleming, Chung, de Jong, Chen, Rice, Bhatia: "In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease." in: Science translational medicine, Vol. 9, Issue 399, (2018) (PubMed).

Banville, Burgess, Jaffar, Tjin, Richeldi, Cerri, Persiani, Black, Oliver: "A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM)." in: PLoS ONE, Vol. 9, Issue 8, pp. e105365, (2014) (PubMed).

Lv, Wu, Zhou, Shao, Wang, Wang: "Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway." in: International journal of endocrinology, Vol. 2014, pp. 658589, (2014) (PubMed).

Xu, Guan, Zheng, Gao, Zeng, Qin, Xue: "Exendin-4 alleviates high glucose-induced rat mesangial cell dysfunction through the AMPK pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 2, pp. 423-32, (2014) (PubMed).

Inoue, Sasaki, Katada, Taguchi: "Quantitative biocompatibility evaluation of nickel-free high-nitrogen stainless steel in vitro/in vivo." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 1, pp. 68-72, (2014) (PubMed).

Zirkel, Lederer, Stöhr, Pazaitis, Hüttelmaier: "IGF2BP1 promotes mesenchymal cell properties and migration of tumor-derived cells by enhancing the expression of LEF1 and SNAI2 (SLUG)." in: Nucleic acids research, Vol. 41, Issue 13, pp. 6618-36, (2013) (PubMed).

Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).

Sasaki, Inoue, Katada, Taguchi: "Promotion of initial cell adhesion on trisuccinimidyl citrate-modified nickel-free high-nitrogen stainless steel." in: Journal of materials science. Materials in medicine, Vol. 24, Issue 4, pp. 951-8, (2013) (PubMed).

Yang, Cheng, Liu, Lu, Song, Jiang, Wu, Guo: "Comparison of mesenchymal stem cells released from poly(N-isopropylacrylamide) copolymer film and by trypsinization." in: Biomedical materials (Bristol, England), Vol. 7, Issue 3, pp. 35003, (2012) (PubMed).

Bai, Zeng, Zhou, Liao, Zhang, Xu, Han, Pei, Liu, Liu, Yao, Xu: "Cdc42-interacting protein-4 promotes TGF-?1-induced epithelial-mesenchymal transition and extracellular matrix deposition in renal proximal tubular epithelial cells." in: International journal of biological sciences, Vol. 8, Issue 6, pp. 859-69, (2012) (PubMed).

Mao, Gao, Li, Li: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, (2011) (PubMed).

Zeng, Han, Luo, Li, Pei, Liao, Bai, Ge, Liu, Xu: "Role of Sema4C in TGF-?1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 4, pp. 1149-56, (2011) (PubMed).

Tang, Song, Shi, Yuan, Li: "Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1." in: Biochimica et biophysica acta, Vol. 1800, Issue 3, pp. 380-4, (2010) (PubMed).

Details zu Fibronectin 1

Target: Fibronectin 1 (FN1)

Andere Bezeichnung: FN1

Hintergrund:

Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Gene Full Name: fibronectin 1

Gen-ID: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie