Caspase 3 ELISA Kit

Produktnummer ABIN416112

Das Ratte Caspase 3 ELISA Kit (ABIN416112) ist ein Colorimetric ELISA Kit zur Detektion von Ratte Caspase 3. Das Kit wurde in 35 Publikationen zitiert.

Kurzübersicht für Caspase 3 ELISA Kit (ABIN416112)

Target: Caspase 3 (CASP3)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails Caspase 3 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3).
No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

Sensitivität: < 0.06 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für Caspase 3 ELISA Kit (ABIN416112)

Al Zaabi, Al Salam, Al Suleimani, Ashique, Manoj, Nemmar, Ali: "Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 394, Issue 2, pp. 249-259, (2021) (PubMed).

Kar, Hacioglu, Kacar, Sahinturk, Kanbak: "Betaine suppresses cell proliferation by increasing oxidative stress-mediated apoptosis and inflammation in DU-145 human prostate cancer cell line." in: Cell stress & chaperones, Vol. 24, Issue 5, pp. 871-881, (2020) (PubMed).

El-Mansi, ElSayyad, Elshershaby, Al-Ashry et al.: "Dietary supplementation of barley and/or dates attenuate hypercholesterolemic-induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic pathways and enhancing oxidative ..." in: Journal of food biochemistry, Vol. 43, Issue 11, pp. e13001, (2020) (PubMed).

Kar, Hacioglu, Senturk, Donmez, Kanbak, Uslu: "Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway." in: Life sciences, Vol. 256, pp. 118016, (2020) (PubMed).

Hao, Su, Sun, Kong, Zhu, Su: "Adiponectin attenuates lipopolysaccharide-induced cell injury of H9c2 cells by regulating AMPK pathway." in: Acta biochimica et biophysica Sinica, Vol. 51, Issue 2, pp. 168-177, (2019) (PubMed).

Shafeek, Abu-Elsaad, El-Karef, Ibrahim: "Gum Acacia mitigates diclofenac nephrotoxicity by targeting monocyte chemoattractant protein-1, complement receptor-1 and pro-apoptotic pathways." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 129, pp. 162-168, (2019) (PubMed).

Shaker, Abboud, Assad, Hadi: "Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis." in: BMC pharmacology & toxicology, Vol. 19, Issue 1, pp. 3, (2019) (PubMed).

Wang, Yan: "A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats." in: International journal of biological macromolecules, Vol. 105, Issue Pt 1, pp. 447-451, (2018) (PubMed).

Shoieb, Esmat, Khalifa, Abdel-Naim: "Chrysin attenuates testosterone-induced benign prostate hyperplasia in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 111, pp. 650-659, (2018) (PubMed).

Ustuner, Colak, Dincer, Tekin, Burukoglu Donmez, Akyuz, Colak, Kolaç, Entok, Ustuner: "Posttreatment Effects of Olea Europaea L. Leaf Extract on Carbon Tetrachloride-Induced Liver Injury and Oxidative Stress in Rats." in: Journal of medicinal food, Vol. 21, Issue 9, pp. 899-904, (2018) (PubMed).

Diling, Tianqiao, Jian, Chaoqun, Ou, Yizhen: "Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus." in: Frontiers in pharmacology, Vol. 8, pp. 219, (2017) (PubMed).

Abd Elwahab, Ramadan, Schaalan, Tolba: "A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 43, Issue 2, pp. 644-659, (2017) (PubMed).

Elsherbiny, Salama, Said, El-Sherbiny, Al-Gayyar: "Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-regulation of inflammatory and apoptic pathways." in: Chemico-biological interactions, Vol. 247, pp. 39-48, (2016) (PubMed).

Colak, Ustuner, Tekin, Colak, Burukoglu, Degirmenci, Gunes: "The hepatocurative effects of Cynara scolymus L. leaf extract on carbon tetrachloride-induced oxidative stress and hepatic injury in rats." in: SpringerPlus, Vol. 5, pp. 216, (2016) (PubMed).

Jiang, Wang, Gu, Wu, Xiao, Chen, Guo, Tang: "Arecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance of Endogenous H2S Generation." in: Neurochemical research, Vol. 41, Issue 8, pp. 2140-8, (2016) (PubMed).

Abd-ElSalam, Al-Ghobashy, Al-Shorbagy, Nassar, Zaazaa, Ibrahim: "Correlation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea Nutraceuticals." in: Journal of food science, Vol. 81, Issue 7, pp. C1707-15, (2016) (PubMed).

Sayed, Saad, El-Sahar: "Dapoxetine attenuates testosterone-induced prostatic hyperplasia in rats by the regulation of inflammatory and apoptotic proteins." in: Toxicology and applied pharmacology, Vol. 311, pp. 52-60, (2016) (PubMed).

El-Tantawy: "Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats." in: Journal of traditional and complementary medicine, Vol. 6, Issue 4, pp. 327-331, (2016) (PubMed).

Ahmed: "Amelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: Effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathway." in: Toxicology and applied pharmacology, Vol. 282, Issue 3, pp. 285-96, (2015) (PubMed).

Youssef, Mahmoud, Abdelghany: "A new combination of sitagliptin and furosemide protects against remote myocardial injury induced by renal ischemia/reperfusion in rats." in: Biochemical pharmacology, Vol. 96, Issue 1, pp. 20-9, (2015) (PubMed).

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: CASP3

Hintergrund: CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1

UniProt: P55213

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation