Angiotensin II ELISA Kit

Produktnummer ABIN414350

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Angiotensin II. Es wurde in 14 Publikationen zitiert.

Kurzübersicht für Angiotensin II ELISA Kit (ABIN414350)

Target: Angiotensin II (Ang II)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 24.69 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Angiotensin II ELISA Kit

Untere Nachweisgrenze: 24.69 pg/mL

Verwendungszweck: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Sensitivität: < 9.44 pg/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für Angiotensin II ELISA Kit (ABIN414350)

El-Ariny, El-Garem, Eid, Donia, Aly, Abdelmeniem: "Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status." in: Andrologia, Vol. 52, Issue 3, pp. e13513, (2020) (PubMed).

Whetton, Preston, Abubeker, Geifman: "Proteomics and Informatics for Understanding Phases and Identifying Biomarkers in COVID-19 Disease." in: Journal of proteome research, Vol. 19, Issue 11, pp. 4219-4232, (2020) (PubMed).

Surcel, Surcel, Zlatescu-Marton, Micu, Nemeti, Axente, Mirza, Neamtiu et al.: "THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE DEVELOPMENT OF SEVERE OVARIAN HYPERSTIMULATION SYNDROME IN PATIENTS WITH PROPHYLACTIC ..." in: Acta endocrinologica (Bucharest, Romania : 2005), Vol. 16, Issue 1, pp. 30-36, (2020) (PubMed).

Montrezol, Marinho, Mota, Dʼalmeida, de Oliveira, Gomes, Medeiros: "ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance Training." in: Journal of strength and conditioning research, Vol. 33, Issue 4, pp. 1119-1129, (2019) (PubMed).

Liu, Li, Wu, Tong, Chen, Zhu, Qian, Chen: "Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes." in: International journal of ophthalmology, Vol. 11, Issue 12, pp. 1889-1894, (2018) (PubMed).

Zheng, Wang, Zou, Jing, Yang, Li, Wang: "Ginsenoside Rb1 improves cardiac function and remodeling in heart failure." in: Experimental animals, Vol. 66, Issue 3, pp. 217-228, (2017) (PubMed).

Jeong, Hong, Cho, Lee, Kwon, Joo, Park, Yu, Lim: "Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 32, Issue 4, pp. 461-7, (2016) (PubMed).

Clancy, Koblar, Golledge: "Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase Regulation in Human Carotid Atheroma in vitro." in: Journal of atherosclerosis and thrombosis, Vol. 23, Issue 7, pp. 773-91, (2016) (PubMed).

Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, (2016) (PubMed).

Chang, Lee, Chen, Lin, Hsu, Chan, Chang, Chang, Pan, Sawamura, Chang, Su, Chen: "Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction." in: Journal of molecular and cellular cardiology, Vol. 84, pp. 36-44, (2015) (PubMed).

Huang, Guo, Sun, Chen, Yin: "Angiotensin II promotes the progression of human gastric cancer." in: Molecular medicine reports, Vol. 9, Issue 3, pp. 1056-60, (2014) (PubMed).

Wang, Sjöberg, Tang, Oörni, Wu, Liu, Secco, Tia, Sukhova, Fernandes, Lesner, Kovanen, Libby, Cheng, Shi: "Cathepsin G activity lowers plasma LDL and reduces atherosclerosis." in: Biochimica et biophysica acta, Vol. 1842, Issue 11, pp. 2174-83, (2014) (PubMed).

Zhang, Long, Sun, Wang, Li, Wu, Guo, Li, Niu, Li, Liu, Mei et al.: "Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by ..." in: European journal of pharmacology, Vol. 651, Issue 1-3, pp. 187-96, (2010) (PubMed).

Wang, Liu, Peng, Tang, Tang, Chen, Lou, Zhang: "Mesangial cells stimulated by immunoglobin A1 from IgA nephropathy upregulates transforming growth factor-beta1 synthesis in podocytes via renin-angiotensin system activation." in: Archives of medical research, Vol. 41, Issue 4, pp. 255-60, (2010) (PubMed).

Details zu Angiotensin II

Target: Angiotensin II (Ang II)

Andere Bezeichnung: AngII

Hintergrund: Ang-II, Angiotensin-2

Pathways: JAK-STAT Signalweg, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity