IL-18 ELISA Kit

Produktnummer ABIN411405

Das Human IL-18 ELISA Kit (ABIN411405) ist ein Colorimetric ELISA Kit zur Detektion von Human IL-18. Das Kit wurde in 8 Publikationen zitiert.

Kurzübersicht für IL-18 ELISA Kit (ABIN411405)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Bindungsspezifität: AA 33-197

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails IL-18 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich Human IL-18 ELISA Kit to quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: Y33-D197

Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.3%, 5.6%, 4.2%
Inter-Assay Precision (CV%): 7.4%, 6.4%, 5.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-18 ELISA Kit (ABIN411405)

Wang, Qin, Wang, Chen, Lang, Zheng, Gao, Chen, Zhong, Mu, Wu, Zhang, Zhao, Zhong: "Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection affects viral replication and host response." in: Scientific reports, Vol. 8, Issue 1, pp. 2887, (2018) (PubMed).

Wan, Yuan, Liu, Xue: "miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells." in: Experimental and therapeutic medicine, Vol. 15, Issue 3, pp. 2429-2435, (2018) (PubMed).

Zhao, Shen, Feng, He, Xu, Xie, Shi, Zhou, Pan, Wang, Guo, Qin: "Regulatory B cells induced by pancreatic cancer cell-derived interleukin-18 promote immune tolerance via the PD-1/PD-L1 pathway." in: Oncotarget, Vol. 9, Issue 19, pp. 14803-14814, (2018) (PubMed).

Esfahani, Saidijam, Najafi, Goodarzi, Movahedian: "The effect of salusin-β on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)." in: ARYA atherosclerosis, Vol. 14, Issue 1, pp. 1-10, (2018) (PubMed).

Trentini, Castellazzi, Cervellati, Manfrinato, Tamborino, Hanau, Volta, Baldi, Kostic, Drulovic, Granieri, Dallocchio, Bellini, Dujmovic, Fainardi: "Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients." in: Disease markers, Vol. 2016, pp. 3672353, (2017) (PubMed).

Huang, Liu, Ma, Liao, Lu, Huang, Qin, Liu, Fang: "Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages." in: Journal of medical virology, (2017) (PubMed).

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

McCarthy: "Management. The name game." in: Nursing times, Vol. 85, Issue 1, pp. 44-5, (1989) (PubMed).

Details zu IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: IL18

Hintergrund:

Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Gene Full Name: interleukin 18

Gen-ID: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome